Comparative Phenotypic Analysis of Anabaena sp. PCC 7120 Mutants of Porinlike Genes
نویسندگان
چکیده
منابع مشابه
Isolation and complementation of nitrogen fixation mutants of the cyanobacterium Anabaena sp. strain PCC 7120.
Approximately 140 mutants of Anabaena sp. strain PCC 7120 unable to grow aerobically on media lacking fixed nitrogen (Fix-) were isolated after mutagenesis with diethyl sulfate and penicillin enrichment. A large cosmid library of wild-type Anabaena sp. strain PCC 7120 DNA was constructed in a mini-RK-2 shuttle vector, and seven mutants representing several morphologically abnormal heterocyst ph...
متن کاملExpression of Shewanella oneidensis MR - 1 [ FeFe ] - hydrogenase 2 genes in Anabaena sp . strain PCC 7120
10 H2 generated from renewable resources holds promise as an environmentally innocuous fuel that 11 releases only energy and water when consumed. In biotechnology, photoautotrophic oxygenic 12 diazotrophs could produce H2 from water and sunlight using the cells’ endogenous nitrogenases. 13 However, nitrogenases have low turnover numbers and require large amounts of ATP. [FeFe]14 hydrogenases fo...
متن کاملExpression of Shewanella oneidensis MR-1 [FeFe]-hydrogenase genes in Anabaena sp. strain PCC 7120.
H(2) generated from renewable resources holds promise as an environmentally innocuous fuel that releases only energy and water when consumed. In biotechnology, photoautotrophic oxygenic diazotrophs could produce H(2) from water and sunlight using the cells' endogenous nitrogenases. However, nitrogenases have low turnover numbers and require large amounts of ATP. [FeFe]-hydrogenases found in oth...
متن کاملNtcA regulates patA expression in Anabaena sp. strain PCC 7120.
patA expression is induced 3 to 6 h after nitrogen step-down. We establish that the transcription of patA is under the positive control of NtcA. The patA promoter region shows two conserved NtcA-binding boxes. These NtcA-binding sites and their interaction with NtcA are key elements for patA expression in heterocysts.
متن کاملAnabaena sp. strain PCC 7120 hetY gene influences heterocyst development.
The filamentous cyanobacterium Anabaena (Nostoc) sp. strain PCC 7120 responds to starvation for fixed nitrogen by producing a semiregular pattern of nitrogen-fixing cells called heterocysts. Overexpression of the hetY gene partially suppressed heterocyst formation, resulting in an abnormal heterocyst pattern. Inactivation of hetY increased the time required for heterocyst maturation and caused ...
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ژورنال
عنوان ژورنال: Journal of Microbiology and Biotechnology
سال: 2021
ISSN: 1017-7825,1738-8872
DOI: 10.4014/jmb.2103.03009